For researchers at the University
and in industry, the Biotechnology Resource Center
offers customized research & development services
as well as limited production services in the following
areas:
If you need advice on refining your experiments or
want us to fully execute your project, our team of experts
will be glad to assist you. Even if your project does
not clearly fall in any of the areas described below,
do not hesitate to contact us, since we are constantly
striving to expand our portfolio of services.
At the Biotechnology Resource Center we have experience
with fermentations using a wide variety of bacteria,
yeast, and fungi. The services we offer include:
- strain development
- media development and optimization
- overall process development
- scale-up
- biomass production
- primary and secondary metabolite production
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The Biotechnology Resource center offers capabilities
for the recovery and purification of cells, proteins,
as well as primary and secondary metabolites from the
bench-scale to the pilot-scale. Services include:
- production of frozen cell pellets
- cell disruption
- inclusion body preparations
- product harvest and concentration by micro- and
ultrafiltration
- chromatographic purification (ion-exchange, affinity,
size exclusion)
- protein refolding
- freeze drying
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Our team of molecular biologists has extensive experience
with bacterial, yeast, animal, and plant genetics. Services
include:
- cloning of genes
- site-directed and random mutagenesis
- sequence verification
- construction of expression vectors
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Our work in protein expression integrates most of the
services described above. Our primary expression hosts
are E. coli, Pichia pastoris, and the
Baculovirus/insect cell system. However, other
organisms can be used as well. A complete protein expression
project would comprise the following steps:
- cloning target gene into chosen expression vector
- creation of fusion proteins, if desired
- incorporating expression vector into expression
host
- initial protein expression analysis on a small scale
- expression optimization specific to host organism
- scale-up of fermentation or cell culture to desired
process size
- development and optimization of protein recovery
and purification strategy
- scale-up of recovery and purification process
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